65. Purified Proteases

Mechanism of intramembrane proteolysis investigated with purified rhomboid proteases.

Intramembrane proteases have the unusual property of cleaving peptide bonds within the lipid bilayer, an environment not obviously suited to a water-requiring hydrolysis reaction. These enzymes include site-2 protease, gamma-secretase/presenilin, signal peptide peptidase and the rhomboids, and they have a wide range of cellular functions. All have multiple transmembrane domains and, because of ...

Functional Studies of Purified Transmembrane Proteases, Omptins, of Yersinia pestis and Salmonelle enterica

4/2006 Kari Kreander A Study on Bacteria-Targeted Screening and in vitro Safety Assessment of Natural Products 5/2006 Gudrun Wahlström From Actin Monomers to Bundles: The Role of Twinfi lin and a-Actinin in Drosophila melanogaster Development 6/2006 Jussi Joensuu Production of F4 Fimbrial Adhesin in Plants: A Model for Oral Porcine Vaccine against Enterotoxigenic Escherichia coli 7/2006 Heikki ...

Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD)

Dendritic cells (DC) are known to present exogenous protein Ag effectively to T cells. In this study we sought to identify the proteases that DC employ during antigen processing. The murine epidermal-derived DC line Xs52, when pulsed with PPD, optimally activated the PPD-reactive Th1 clone LNC.2F1 as well as the Th2 clone LNC.4k1, and this activation was completely blocked by chloroquine pretre...

Viral proteases.

Viruses are major pathogenic agents that can cause a variety of serious diseases in humans, other animals, and plants. Due to their clinical and scientific importance, viruses have been under intensive study ever since their first isolation about a century ago. The studies of viruses, their life cycles, and their interactions with the hosts have established many new areas of biological research...

Proteases in the Brain Proteases in Biology and Disease